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Behavior

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JoVE Journal Behavior
Um ensaio de buraqueira/Tunneling para deteção de hipóxia em larvas de Drosophila melanogaster
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Um ensaio de buraqueira/Tunneling para deteção de hipóxia em larvas de Drosophila melanogaster

Article DOI: 10.3791/57131-v • 09:04 min • March 27th, 2018
March 27th, 2018 •
1,2, 1, 1
1Department of Biosciences, Rice University, 2Yale Medical School

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O protocolo descreve um ensaio simples para identificar larvas de Drosophila melanogaster que estão enfrentando a hipóxia sob os níveis de oxigénio atmosférico normal. Este protocolo permite hipóxicos larvas ser distinguido de outros mutantes que mostram fenótipos sobrepostos como lentidão ou crescimento lento.

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Comportamento edição 133 d. melanogaster larva hipóxia comida cavando substratos de encapsulamento crescimento larval traqueia locomoção larval sem fôlego uninflatable
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