JoVE Journal
Chemistry
Chemistry
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シリアル結晶の結晶 x 線回折その場その場での動的光散乱のためのマイクロ流体チップ
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Article
DOI:
10.3791/57133-v
•
11:48 min
•
April 24th, 2018
シリアル結晶の結晶 x 線回折その場その場での動的光散乱のためのマイクロ流体チップ
April 24th, 2018
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Yannig Gicquel*1,2, Robin Schubert*3,4,5, Svetlana Kapis3, Gleb Bourenkov6, Thomas Schneider6, Markus Perbandt3,4, Christian Betzel3,4,5, Henry N. Chapman1,2,4, Michael Heymann1,7
1Center for Free Electron Laser Science, DESY, 2Department of Physics, University of Hamburg, 3Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation, University of Hamburg, 4The Hamburg Center for Ultrafast Imaging, University of Hamburg, 5Integrated Biology Infrastructure Life-Science Facility at the European XFEL (XBI), 6European Molecular Biology Laboratory, EMBL c/o DESY, 7Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry
* These authors contributed equally
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
このプロトコルでは、作製し、室温での x 線回折データ収集のためのマイクロ流体デバイスを操作する方法について詳しく説明します。さらに、動的光散乱法によるタンパク質の結晶化を監視する方法を説明し、処理し、分析する方法得られた回折データ。