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Journal / Chemistry / Mikrofluidische Chips für In Situ…
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Chemistry

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JoVE Journal Chemistry
Mikrofluidische Chips für In Situ Crystal-Röntgenbeugung und In Situ dynamische Lichtstreuung für serielle Kristallographie
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Mikrofluidische Chips für In Situ Crystal-Röntgenbeugung und In Situ dynamische Lichtstreuung für serielle Kristallographie

Article DOI: 10.3791/57133-v • 11:48 min • April 24th, 2018
April 24th, 2018 •
*1,2, *3,4,5, 3, 6, 6, 3,4, 3,4,5, 1,2,4, 1,7
1Center for Free Electron Laser Science, DESY, 2Department of Physics, University of Hamburg, 3Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation, University of Hamburg, 4The Hamburg Center for Ultrafast Imaging, University of Hamburg, 5Integrated Biology Infrastructure Life-Science Facility at the European XFEL (XBI), 6European Molecular Biology Laboratory, EMBL c/o DESY, 7Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry
* These authors contributed equally

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Dieses Protokoll beschreibt im Detail wie fabrizieren und mikrofluidischen Geräte zur Röntgenbeugung Datenerfassung bei Raumtemperatur betrieben. Darüber hinaus beschreibt die Kristallisation von Proteinen durch dynamische Lichtstreuung zu überwachen und Informationen zum Verarbeiten und analysieren erhalten Beugung Daten.

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Chemie Ausgabe 134 in Situ Röntgendiffraktometrie feste Ziel serielle Millisekunde Kristallographie Mikrofluidik Protein Kristallisation in Situ dynamischen Licht Streuung
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