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Journal / Chemistry / Microfluidic brikker for In Situ Crystal X-ray…
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Chemistry

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JoVE Journal Chemistry
Microfluidic brikker for In Situ Crystal X-ray Diffraksjon og In Situ dynamisk lysspredning for føljetong krystallografi
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Microfluidic brikker for In Situ Crystal X-ray Diffraksjon og In Situ dynamisk lysspredning for føljetong krystallografi

Article DOI: 10.3791/57133-v • 11:48 min • April 24th, 2018
April 24th, 2018 •
*1,2, *3,4,5, 3, 6, 6, 3,4, 3,4,5, 1,2,4, 1,7
1Center for Free Electron Laser Science, DESY, 2Department of Physics, University of Hamburg, 3Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation, University of Hamburg, 4The Hamburg Center for Ultrafast Imaging, University of Hamburg, 5Integrated Biology Infrastructure Life-Science Facility at the European XFEL (XBI), 6European Molecular Biology Laboratory, EMBL c/o DESY, 7Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry
* These authors contributed equally

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Denne protokollen beskriver i detalj hvordan du dikte og microfluidic enhetene for X-ray Diffraksjon datainnsamling ved romtemperatur. I tillegg beskriver hvordan overvåke protein krystallisering av dynamisk lysspredning og hvordan å behandle og analysere fikk Diffraksjon data.

Tags

Kjemi problemet 134 i situ X-ray Diffraksjon fast mål seriell millisekund krystallografi microfluidics protein krystallisering i situ dynamisk lys spredning
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