Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / En Optogenetic metode for å styre og analysere…
JoVE Journal
Genetics

A subscription to JoVE is required to view this content.

JoVE Journal Genetics
En Optogenetic metode for å styre og analysere Gene Expression mønstre i celle til celle interaksjoner
Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes
Journal (Genetics)
Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes
Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
Journal (Genetics)
Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
 
Click here for the English version

En Optogenetic metode for å styre og analysere Gene Expression mønstre i celle til celle interaksjoner

Article DOI: 10.3791/57149-v • 07:59 min • March 22nd, 2018
March 22nd, 2018 •
1,2, 1,3,4,5
1Institute for Frontier Life and Medical Sciences, Kyoto University, 2Japan Science and Technology Agency, PRESTO, 3Institute for Integrated Cell-Material Sciences, Kyoto University, 4Graduate School of Medicine, Kyoto University, 5Graduate School of Biostudies, Kyoto University

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Her presenterer vi en protokoll for å analysere celle til celle overføring av oscillasjon informasjon av optogenetic kontroll og live overvåking av genuttrykk. Denne tilnærmingen gir en unik plattform for å teste en funksjonell betydning av dynamisk gene expression programmer i flercellet systemer.

Tags

Genetikk problemet 133 optogenetic metoden sanntid bildebehandling luciferase reporter GAVPO oscillasjon uttrykk hakk signalisering
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter