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Journal / Immunology and Infection / Histologische kwantificering te bepalen van de…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Histologische kwantificering te bepalen van de schimmel last long in experimentele aspergillose
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Histologische kwantificering te bepalen van de schimmel last long in experimentele aspergillose

Article DOI: 10.3791/57155-v • 09:52 min • March 9th, 2018
March 9th, 2018 •
1, 1, 1, 1, 1
1Department of Microbiology and Immunology, Indiana University School of Medicine - Terre Haute

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Hier beschrijven we een protocol om te bepalen van pulmonaire schimmel last in muizen met invasieve aspergillose door kwantificering van Gomori van gemodificeerde methanamine zilver kleuring in histologische secties. Gebruik van deze methode resulteerde in vergelijkbare resultaten met minder dieren in vergelijking met de beoordeling van schimmel last van kwantitatieve PCR van longkanker schimmel DNA.

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Immunologie kwestie 133 Aspergillus fumigatus Long schimmel last histologie GMS kwantificering kwantitatieve PCR aspergillose
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