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JoVE Journal Developmental Biology
Tre-dimensionelle inflammatoriske humant væv ækvivalenter af Gingiva
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Tre-dimensionelle inflammatoriske humant væv ækvivalenter af Gingiva

Article DOI: 10.3791/57157-v • 08:43 min • April 3rd, 2018
April 3rd, 2018 •
1, 2, 2
1Department of Pharmacology, The Nippon Dental University, 2Department of Oral and Maxillofacial Surgery, The Nippon Dental University Hospital

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Målet med protokollen er at opbygge en inflammatorisk menneskelige gingiva model in vitro. Dette væv model dyrker Co tre typer af humane celler, HaCaT keratinocytter, gingival fibroblaster og THP-1 makrofager, tre-dimensionelle betingelser. Denne model kan anvendes til at undersøge parodontale sygdomme, såsom gingivitis og parodontitis.

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Udviklingsmæssige biologi spørgsmål 134 tre-dimensionelle kultur gingiva ækvivalenter menneskelige gingival fibroblaster HaCaT celler THP-1 celler makrofager vævsmanipulering inflammatoriske væv model gingivitis parodontitis sårheling væv regenerering
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