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Journal / Biology / Konfokal mikroskopi afslører celle overflade…
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JoVE Journal Biology
Konfokal mikroskopi afslører celle overflade Receptor sammenlægning gennem billedet korrelation spektroskopi
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Konfokal mikroskopi afslører celle overflade Receptor sammenlægning gennem billedet korrelation spektroskopi

Article DOI: 10.3791/57164-v • 06:51 min • August 2nd, 2018
August 2nd, 2018 •
1,2, 3, 4, 1,2,5,6,7
1Tumour Targeting Laboratory, Olivia Newton-John Cancer Research Institute, 2School of Cancer Medicine, La Trobe University, 3Centre for Micro-Photonics, Faculty of Science, Engineering and Technology, Swinburne University of Technology, 4LIMS Bioimaging Facility, La Trobe Institute for Molecular Science, La Trobe University, 5Department of Medical Oncology, Olivia Newton-John Cancer and Wellnes Centre, Austin Health, 6Department of Medicine, University of Melbourne, 7Department of Molecular Imaging and Therapy, Austin Health

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Antistoffer, der binder til at målrette receptorer på cellens overflade kan tillægge kropsbygning og klyngedannelse ændringer. Disse dynamiske ændringer har konsekvenser for kendetegner stof udvikling i target-cellerne. Denne protokol udnytter Konfokal mikroskopi og billede korrelation spektroskopi gennem ImageJ/FIJI at opgøre omfanget af receptor klynger på cellens overflade.

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Biologi spørgsmålet 138 Konfokal mikroskopi billede korrelation spektroskopi (ICS) antistof: receptor interaktioner antistof-stof-konjugat (ADC) imageJ Fiji image processing klyngedannelse sammenlægning
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