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Journal / Chemistry / Captação de Metal essencial em bactérias…
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Chemistry

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JoVE Journal Chemistry
Captação de Metal essencial em bactérias Gram-negativas
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Captação de Metal essencial em bactérias Gram-negativas: fluorescência, radioisótopos, um raio-x e fracionamento da pilha

Article DOI: 10.3791/57169-v • 10:34 min • February 1st, 2018
February 1st, 2018 •
1, 2, 2, 3, 2, 4
1Graduate Biomedical Sciences Microbiology Theme, University of Alabama at Birmingham, 2Department of Radiology, University of Alabama at Birmingham, 3Office of the Provost, University of Alabama at Birmingham, 4Department of Pharmacology and Toxicology, University of Alabama at Birmingham

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Um protocolo para a extração de um acompanhante de metal de transição periplasmático no contexto dos seus parceiros de ligação nativo e caracterização Biofísica de seu conteúdo de substrato por raios-x, absorção fluorescência e radiometal é apresentada.

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Química questão 132 fracionamento Periplasm fluorescência de raios-x Metal de transição proteína ligadora de substrato (SBP) YfeA Yersinia pestis Praga Radiotracer radioatividade
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