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Journal / Biochemistry / Dannelsen af kovalent DNA adukter af enzymatisk…
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Biochemistry

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JoVE Journal Biochemistry
Dannelsen af kovalent DNA adukter af enzymatisk aktiveret kræftfremkaldende stoffer og narkotika In Vitro og deres bestemmelse af 32P-postlabeling
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Dannelsen af kovalent DNA adukter af enzymatisk aktiveret kræftfremkaldende stoffer og narkotika In Vitro og deres bestemmelse af 32P-postlabeling

Article DOI: 10.3791/57177-v • 09:33 min • March 20th, 2018
March 20th, 2018 •
1
1Department of Biochemistry, Charles University

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Vurdere styrken af miljømæssige kemikalier og lægemidler, er enzymatisk bioactivated til mellemprodukter generere kovalente DNA adukter, er et vigtigt felt i udviklingen af kræft og dens behandling. Metoder er beskrevet for sammensatte aktivering til at danne DNA adukter, samt teknikker til deres påvisning og kvantificering.

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Biokemi sag 133 narkotika kræftfremkaldende bioactivation,cytokrom P450 reductases DNA adukter 32P-postlabeling
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