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Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Article DOI: 10.3791/57188-v • 11:48 min • May 31st, 2018
May 31st, 2018 •
*1, *1, 1, 1, 1, 2, 1
1Department of Pathology, Sackler School of Medicine, Tel-Aviv University, 2Department of Pathology, School of Medicine, Stanford University
* These authors contributed equally

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Summary
Automatic Translation

Monocyte-derived DC (MoDC) can sense minor amounts of danger-associated molecules and are therefore easily primed. We provide a detailed protocol for the isolation of MoDC from blood and tumors and their activation with immune complexes while highlighting key precautions that should be considered in order to avoid their premature activation.

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Isolation Protocol Mouse Monocyte-derived Dendritic Cells In Vitro Activation Tumor Immune Complexes Immunostimulatory Properties Activation State Sterile Reagents Contamination Myeloid Cells Dendritic Cells Patterns Of Cell Activation Procedure Demonstration Euthanizing Mice Carbon Dioxide Laminar Flow Hood RPMI Medium Fetal Bovine Serum Surgery Scissors Tumor Pieces 30-milliliter Flat-bottom Tube HBSS Buffer Collagenase IV DNase I Magnetic Stir Bars Incubation Temperature Revolutions Per Minute
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