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Developmental Biology

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JoVE Journal Developmental Biology
Identifikation af skeletmuskulatur satellit celler ved immunfluorescens med Pax7 og Laminin antistoffer
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Identifikation af skeletmuskulatur satellit celler ved immunfluorescens med Pax7 og Laminin antistoffer

Article DOI: 10.3791/57212-v • 07:18 min • April 19th, 2018
April 19th, 2018 •
*1, *2, 1, 2, 1
1Laboratory of Muscle Stem Cells and Gene Regulation, National Institutes of Health, 2Office of Science and Technology, National Institute of Arthritis, Musculoskeletal and Skin Diseases (NIAMS), National Institutes of Health
* These authors contributed equally

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Nøjagtig identifikation af satellit celler er afgørende for at studere deres funktioner under forskellige fysiologiske og patologiske betingelser. Denne artikel præsenterer en protokol for at identificere satellit celler på voksne skeletmuskulatur sektioner af immunofluorescens-baserede farvning.

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Udviklingsmæssige biologi spørgsmål 134 skeletmuskulatur satellit celler Pax7 immunofluorescens stamceller basal lamina
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