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Journal / Neuroscience / Optogenetics combineren met kunstmatige microRNAs …
JoVE Journal
Neuroscience

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JoVE Journal Neuroscience
Optogenetics combineren met kunstmatige microRNAs te karakteriseren van de effecten van Gene Knockdown op presynaptische functie binnen Intact neuronale Circuits
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Optogenetics combineren met kunstmatige microRNAs te karakteriseren van de effecten van Gene Knockdown op presynaptische functie binnen Intact neuronale Circuits

Article DOI: 10.3791/57223-v • 09:17 min • March 14th, 2018
March 14th, 2018 •
1, 1, 1
1Center for Synaptic Neuroscience and Technology, Istituto Italiano di Tecnologia

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Dit protocol biedt een workflow op hoe te combineren met kunstmatige microRNA-gemedieerde RNA-interferentie met optogenetics te stimuleren specifiek presynaptische los met verminderde expressie van selectieve gene(s) binnen intact neuronale circuits.

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Neurowetenschappen kwestie 133 Optogenetics ultrasnelle channelrhodopsin ChETA microRNA RNA-interferentie knockdown recombinante adeno-associated virus rAAV1/2 presynaptische synaptische transmissie synaptische plasticiteit RNA extractie
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