10,113 Views
•
09:17 min
•
March 14, 2018
DOI:
Transcript
Automatically generated
Summary
Automatically generated
Ce protocole prévoit un flux de travail sur la façon de combiner artificielle induite sur les micro-ARN interférence d’ARN avec optogenetics pour stimuler spécifiquement présynaptiques boutons avec une expression réduite de sélectives ou des gènes au sein des circuits de neurones intacts.
Related Videos
09:50
Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation
Related Videos
17175 Views
12:13
Multi-unit Recording Methods to Characterize Neural Activity in the Locust (Schistocerca Americana) Olfactory Circuits
Related Videos
26930 Views
10:07
In Vivo Two-photon Imaging Of Experience-dependent Molecular Changes In Cortical Neurons
Related Videos
21532 Views
07:08
Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration
Related Videos
19378 Views
10:58
A Procedure for Implanting Organized Arrays of Microwires for Single-unit Recordings in Awake, Behaving Animals
Related Videos
13167 Views
10:26
Rewiring Neuronal Circuits: A New Method for Fast Neurite Extension and Functional Neuronal Connection
Related Videos
8675 Views
07:33
Optogenetic Entrainment of Hippocampal Theta Oscillations in Behaving Mice
Related Videos
11606 Views
07:51
Optogenetics Identification of a Neuronal Type with a Glass Optrode in Awake Mice
Related Videos
6688 Views
08:51
In Vivo Calcium Imaging of Lateral-line Hair Cells in Larval Zebrafish
Related Videos
11284 Views
08:16
Combining Behavior and EEG to Study the Effects of Mindfulness Meditation on Episodic Memory
Related Videos
6923 Views
Read Article
Cite this Article
Thalhammer, A., Jaudon, F., Cingolani, L. A. Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits. J. Vis. Exp. (133), e57223, doi:10.3791/57223 (2018).
Copy