JoVE Journal
Bioengineering
Bioengineering
A subscription to JoVE is required to view this content.
Chapters
Summary
The protocol describes how to engineer a perfusable vascular network in a spheroid. The spheroid's surrounding microenvironment is devised to induce angiogenesis and connect the spheroid to the microchannels in a microfluidic device. The method allows the perfusion of the spheroid, which is a long-awaited technique in three-dimensional cultures.