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Journal
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Developmental Biology
/
致心律失常心肌病患者心内膜心肌活检样间质基质细胞的分离与鉴定
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients
Please note that all translations are automatically generated.
Click here for the English version.
致心律失常心肌病患者心内膜心肌活检样间质基质细胞的分离与鉴定
DOI:
10.3791/57263-v
•
09:16 min
•
February 28, 2018
•
Chiara Assunta Pilato*
1,2
,
Ilaria Stadiotti*
1
,
Angela Serena Maione
,
Valentina Saverio
,
Valentina Catto
,
Fabrizio Tundo
,
Antonio Dello Russo
,
Claudio Tondo
3
,
Giulio Pompilio
2
,
Michela Casella*
3
,
Elena Sommariva*
1
1
Vascular Biology and Regenerative Medicine Unit
,
Centro Cardiologico Monzino-IRCCS
,
2
Department of Clinical Sciences and Community Health
,
Università degli Studi di Milano
,
3
Heart Rhythm Center
,
Centro Cardiologico Monzino-IRCCS
Chapters
00:04
Title
01:12
Cardiac Biopsy Collection and Processing
03:06
Characterizing Cardiac Mesenchymal Stromal Cells (C-MSC) by Flow Cytometry
05:22
Oil Red O (ORO) Staining C-MSC after Adipogenic Differentiation
07:02
Results: Flow Cytometry Analysis and ORO Staining
08:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文提供了一种从致心律失常心肌病患者心内膜心肌活检样品中分离心间质基质细胞的方法。描述了它们的表征和提高脂肪分化的协议。
Tags
Cardiac Mesenchymal Stromal Cells
Arrhythmogenic Cardiomyopathy
Endomyocardial Biopsy
Adipogenic Differentiation
Phenotyping
Cell Isolation
Cell Culture
Collagenase Digestion
Surface Markers
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