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A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

Article DOI: 10.3791/57271-v • 06:56 min • March 10th, 2018
March 10th, 2018 •
1, 1, 1, 1, 1
1Laboratory of Virology and Chemotherapy, Department of Microbiology and Immunology, Rega Institute for Medical Research, KU Leuven

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Summary
Automatic Translation

A flow cytometry-based cellular binding assay is described that is primarily used as a screening tool to identify compounds that inhibit the binding of a fluorescently labeled CXC chemokine ligand 12 (CXCL12) to the CXC chemokine receptor 4 (CXCR4).

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Flow Cytometry-based Assay Compounds Disrupt Binding Fluorescently-labeled CXC Chemokine Ligand 12 CXC Chemokine Receptor 4 Cell-based Assay G-protein Coupled Receptor Competition Binding Assay Drug Discovery Living Cells Radioactivity Label Probes Chemokine Receptor CXCR4 Fluorescently-labeled Ligands Compound Solution 96 Well Rounded Bottom Plate Cell Suspension Multi-channel Pipette Incubate Room Temperature Dark Centrifuge
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