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Journal / Genetics / Højeffektive gen afbrydelse af Murine og…
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Genetics

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JoVE Journal Genetics
Højeffektive gen afbrydelse af Murine og menneskelige hæmatopoietisk stamceller ved CRISPR/Cas9
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Højeffektive gen afbrydelse af Murine og menneskelige hæmatopoietisk stamceller ved CRISPR/Cas9

Article DOI: 10.3791/57278-v • 08:27 min • April 10th, 2018
April 10th, 2018 •
*1,2,3, *1,2,4, 4, 1,2,4, 1,2,4,5
1Stem Cells & Regenerative Medicine Center, Baylor College of Medicine, 2Center for Cell and Gene Therapy, Baylor College of Medicine, 3Centro di Ricerca Emato-Oncologica (CREO), University of Perugia, 4Department of Molecular & Human Genetics, Baylor College of Medicine, 5Texas Children's Hospital & Houston Methodist Hospital
* These authors contributed equally

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En protokol for hurtig CRISPR/Cas9-medieret gen forstyrrelser i mus og primære hæmatopoietisk menneskeceller er beskrevet i denne artikel. Cas9-sgRNA ribonucleoproteins er indført via elektroporation med sgRNAs genereret gennem in vitro- transskription og kommercielle Cas9. Høj redigering effektivitet opnås med begrænset tid og finansielle omkostninger.

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Genetik spørgsmål 134 CRISPR-Cas9 HSPCs hæmatopoietisk stilk og stamfader celler knock-out ribonucleoprotein gen redigering,
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