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Journal / Genetics / Svært effektiv Gene avbrudd i Murine og…
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Genetics

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JoVE Journal Genetics
Svært effektiv Gene avbrudd i Murine og menneskelig blodkreft stamfar celler av CRISPR/Cas9
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Svært effektiv Gene avbrudd i Murine og menneskelig blodkreft stamfar celler av CRISPR/Cas9

Article DOI: 10.3791/57278-v • 08:27 min • April 10th, 2018
April 10th, 2018 •
*1,2,3, *1,2,4, 4, 1,2,4, 1,2,4,5
1Stem Cells & Regenerative Medicine Center, Baylor College of Medicine, 2Center for Cell and Gene Therapy, Baylor College of Medicine, 3Centro di Ricerca Emato-Oncologica (CREO), University of Perugia, 4Department of Molecular & Human Genetics, Baylor College of Medicine, 5Texas Children's Hospital & Houston Methodist Hospital
* These authors contributed equally

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En protokoll for rask CRISPR/Cas9-mediert genet avbrudd i musen og menneskelige primære blodkreft cellene er beskrevet i denne artikkelen. Cas9-sgRNA ribonucleoproteins blir introdusert via electroporation sgRNAs generert gjennom i vitro transkripsjon og kommersielle Cas9. Høy redigering effektivitet oppnås med begrenset tid og finansielle kostnader.

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Genetikk problemet 134 CRISPR-Cas9 HSPCs blodkreft stilk og stamfar celler knock-out ribonucleoprotein gen redigering,
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