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Journal / Genetics / Disrupção do Gene altamente eficiente de células…
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Genetics

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JoVE Journal Genetics
Disrupção do Gene altamente eficiente de células progenitoras hematopoiéticas murino e humano por CRISPR/Cas9
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Disrupção do Gene altamente eficiente de células progenitoras hematopoiéticas murino e humano por CRISPR/Cas9

Article DOI: 10.3791/57278-v • 08:27 min • April 10th, 2018
April 10th, 2018 •
*1,2,3, *1,2,4, 4, 1,2,4, 1,2,4,5
1Stem Cells & Regenerative Medicine Center, Baylor College of Medicine, 2Center for Cell and Gene Therapy, Baylor College of Medicine, 3Centro di Ricerca Emato-Oncologica (CREO), University of Perugia, 4Department of Molecular & Human Genetics, Baylor College of Medicine, 5Texas Children's Hospital & Houston Methodist Hospital
* These authors contributed equally

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Um protocolo para rápido CRISPR/Cas9-mediada disrupção do gene no mouse e células hematopoiéticas primárias humanas é descrito neste artigo. Ribonucleoproteínas Cas9-sgRNA são introduzidas através de eletroporação com sgRNAs gerados através da transcrição em vitro e Cas9 comercial. Alta eficiência de edição é obtida com tempo limitado e custo financeiro.

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Genética edição 134 CRISPR-Cas9 HSPCs células estaminais e progenitoras hematopoiéticas nocaute ribonucleoprotein gene edição,
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