Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Fare ve insan hematopoetik progenitör hücre…
JoVE Journal
Genetics

This content is Open Access.

JoVE Journal Genetics
Fare ve insan hematopoetik progenitör hücre tarafından CRISPR/Cas9 yüksek verimli Gene bozulma
Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture (4C)
Journal (Genetics)
Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture (4C)
Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates (Video) | JoVE
Journal (Genetics)
Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates (Video) | JoVE
 
Click here for the English version

Fare ve insan hematopoetik progenitör hücre tarafından CRISPR/Cas9 yüksek verimli Gene bozulma

Article DOI: 10.3791/57278-v • 08:27 min • April 10th, 2018
April 10th, 2018 •
*1,2,3, *1,2,4, 4, 1,2,4, 1,2,4,5
1Stem Cells & Regenerative Medicine Center, Baylor College of Medicine, 2Center for Cell and Gene Therapy, Baylor College of Medicine, 3Centro di Ricerca Emato-Oncologica (CREO), University of Perugia, 4Department of Molecular & Human Genetics, Baylor College of Medicine, 5Texas Children's Hospital & Houston Methodist Hospital
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Bir iletişim kuralı için hızlı fare CRISPR/Cas9-aracılı gene bozulma ve insan birincil hematopoetik hücreler bu makalede açıklanan. Cas9-sgRNA ribonucleoproteins elektroporasyon ile vitro transkripsiyon ve ticari Cas9 ile oluşturulan sgRNAs ile tanıtılmaktadır. Yüksek Düzenleme verimliliği sınırlı zaman ve maliyet ile elde edilir.

Tags

Genetik sayı: 134 CRISPR-Cas9 HSPCs hematopoetik kök ve progenitör hücre knock-out Ribonükleoprotein gen düzenleme,
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter