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JoVE Journal Biochemistry
Lipid-Index Bestimmung von flüssigen Fluoreszenz Erholung der pilzliche Erreger Ustilago Maydis
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Journal (Biochemistry)
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Lipid-Index Bestimmung von flüssigen Fluoreszenz Erholung der pilzliche Erreger Ustilago Maydis

Article DOI: 10.3791/57279-v • 10:22 min • April 3rd, 2018
April 3rd, 2018 •
*1,2, *3, 2, 1
1Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 2Facultad de Medicina, Universidad Nacional Autónoma de México, 3Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro
* These authors contributed equally

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Hier beschreiben wir ein Protokoll, um der Lipid-Tröpfchen (LD Index) erreichen, um die Dynamik der Triacylglyzerole in Zellen im Hochdurchsatz-Experimente zu studieren. Die LD-Index-Assay ist eine einfache und zuverlässige Methode, die BODIPY 493/503 verwendet. Dieser Assay benötigt keine Dispendious Lipid-Extraktion oder Mikroskopie-Analyse.

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Biochemie Ausgabe 134 Lipid-Index Lipid-Tröpfchen Basidiomycete Fluoreszenz Erholung BODIPY 493/503 Ustilago maydis
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