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Journal / Genetics / Eine Methode, um den C924T-Polymorphismus von…
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Genetics

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JoVE Journal Genetics
Eine Methode, um den C924T-Polymorphismus von Thromboxan-A2-Rezeptor-Gens zu studieren
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Eine Methode, um den C924T-Polymorphismus von Thromboxan-A2-Rezeptor-Gens zu studieren

Article DOI: 10.3791/57289-v • 07:00 min • April 1st, 2019
April 1st, 2019 •
1, 1, 2, 2, 2, 1, 3, 1, 2, 4, 1,2
1SS Annunziata University Hospital, Unit of Clinical Molecular Biology and Predictive Medicine, University of Chieti, 2Department of Medical, Oral and Biotechnological Sciences, University of Chieti, 3Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, 4CNR - Institute of Molecular Genetics, Section of Chieti

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In der vorliegenden Studie, beschreiben wir eine Methodik zur Analyse des Genotyps C924T. Das Protokoll besteht aus drei Phasen: DNA-Extraktion, Verstärkung durch Polymerase-Kettenreaktion (PCR) und Analyse von der Beschränkung Fragment-Länge Polymorphismus (RFLP) auf Agarosegel.

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Genetik Ausgabe 146 Molekularbiologie Genexpression Restriktionsenzym Analyse Single Nucleotide Polymorphismus Genotypisierung Thrombozytenaggregation Atherothrombosis
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