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Immunology and Infection

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JoVE Journal Immunology and Infection
Replicación de la ordena, no redundante biblioteca de Pseudomonas aeruginosa cepa PA14 mutantes de inserción de transposones
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Replicación de la ordena, no redundante biblioteca de Pseudomonas aeruginosa cepa PA14 mutantes de inserción de transposones

Article DOI: 10.3791/57298-v • 11:35 min • May 4th, 2018
May 4th, 2018 •
*1, *1, 2, 1, 1, 2,3, 1,4, 1,4
1Department of Pediatrics, Mucosal Immunology and Biology Research Center, Massachusetts General Hospital, 2Department of Molecular Biology, Massachusetts General Hospital, 3Department of Genetics, Harvard Medical School, 4Department of Pediatrics, Harvard Medical School
* These authors contributed equally

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Infección por Pseudomonas aeruginosa causa una morbilidad significativa en hosts vulnerables. La biblioteca mutantes de inserción de transposones no redundante de p. aeruginosa cepa PA14, designados como establece PA14NR, facilita el análisis de la funcionalidad del gen en numerosos procesos. Presentamos es un protocolo para generar copias de alta calidad de la biblioteca mutante PA14NR establecido.

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Inmunología e infección número 135 Pseudomonas aeruginosa transposón mariner biblioteca mutante mutagénesis microbiología infección oportunista Inmunología
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