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JoVE Journal Immunology and Infection
Replicatie van de besteld, Nonredundant bibliotheek van Pseudomonas aeruginosa stam PA14 Transposon insertiemutanten
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Replicatie van de besteld, Nonredundant bibliotheek van Pseudomonas aeruginosa stam PA14 Transposon insertiemutanten

Article DOI: 10.3791/57298-v • 11:35 min • May 4th, 2018
May 4th, 2018 •
*1, *1, 2, 1, 1, 2,3, 1,4, 1,4
1Department of Pediatrics, Mucosal Immunology and Biology Research Center, Massachusetts General Hospital, 2Department of Molecular Biology, Massachusetts General Hospital, 3Department of Genetics, Harvard Medical School, 4Department of Pediatrics, Harvard Medical School
* These authors contributed equally

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Pseudomonas aeruginosa infectie veroorzaakt significante morbiditeit in kwetsbare hosts. De nonredundant transposon invoeging mutant bibliotheek van P. aeruginosa stam PA14, uitgeroepen tot PA14NR ingesteld, vergemakkelijkt de analyse van gene functionaliteit in tal van processen. Hier gepresenteerd is een protocol voor het genereren van hoge kwaliteit kopieën van de PA14NR ingesteld mutant bibliotheek.

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Immunologie en infecties probleem 135 Pseudomonas aeruginosa mariner transposon mutant bibliotheek mutagenese microbiologie opportunistische infectie immunologie
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