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Journal / Immunology and Infection / Replikering av beställt, Nonredundant bibliotek…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Replikering av beställt, Nonredundant bibliotek av Pseudomonas aeruginosa stam PA14 Transposon införande mutanter
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Replikering av beställt, Nonredundant bibliotek av Pseudomonas aeruginosa stam PA14 Transposon införande mutanter

Article DOI: 10.3791/57298-v • 11:35 min • May 4th, 2018
May 4th, 2018 •
*1, *1, 2, 1, 1, 2,3, 1,4, 1,4
1Department of Pediatrics, Mucosal Immunology and Biology Research Center, Massachusetts General Hospital, 2Department of Molecular Biology, Massachusetts General Hospital, 3Department of Genetics, Harvard Medical School, 4Department of Pediatrics, Harvard Medical School
* These authors contributed equally

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Pseudomonas aeruginosa infektion orsakar betydande sjuklighet i utsatta värdar. Nonredundant transposon införande mutant biblioteket av P. aeruginosa stam PA14, betecknas som PA14NR inställd, underlättar analys av genen funktionalitet i flera olika processer. Presenteras här är ett protokoll för att skapa högkvalitativa kopior av PA14NR ställa mutant biblioteket.

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Immunologi och infektion fråga 135 Pseudomonas aeruginosa mariner transposon mutant bibliotek mutagenes mikrobiologi opportunistiska infektioner immunologi
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