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Journal / Developmental Biology / Införliva en endotelceller pärla groning Assay…
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JoVE Journal Developmental Biology
Införliva en endotelceller pärla groning Assay pericyter
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Införliva en endotelceller pärla groning Assay pericyter

Article DOI: 10.3791/57309-v • 06:21 min • February 16th, 2018
February 16th, 2018 •
1, 2, 2, 2,3
1Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, 2Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 3Department of Medicine, University of North Carolina at Chapel Hill

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Detta protokoll presenterar en roman i vitro pärla assay som mer modeller på lämpligt sätt processen för i vivo groning angiogenes genom att införliva pericyter. Denna ändring gör det möjligt för bead analysen till mer troget recapitulate heterotypic cellulära interaktioner mellan endotelceller och väggmålning celler som är kritiska för angiogenes.

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Utvecklingsbiologi fråga 132 Endothelial celler HUVEC pericyter Sprouting angiogenes pärla Assay väggmålning celler
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