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Journal / Developmental Biology / Einbeziehung der Perizyten in einem…
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JoVE Journal Developmental Biology
Einbeziehung der Perizyten in einem Endothelzellen Korn sprießen Assay
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Einbeziehung der Perizyten in einem Endothelzellen Korn sprießen Assay

Article DOI: 10.3791/57309-v • 06:21 min • February 16th, 2018
February 16th, 2018 •
1, 2, 2, 2,3
1Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, 2Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 3Department of Medicine, University of North Carolina at Chapel Hill

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Dieses Protokoll stellt eine neuartige in-vitro- Perle assay, die mehr entsprechend modelliert den Prozess der in Vivo Angiogenese durch den Einbau von Perizyten sprießen. Diese Änderung ermöglicht die Wulst-Assay, treuer rekapitulieren die Zellkulturmodells zellulären Interaktionen zwischen Endothelzellen und Wandbild Zellen, die entscheidend für die Angiogenese.

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Entwicklungsbiologie Ausgabe 132 Endothelial Zellen HUVECS Perizyten entspringen Angiogenese Bead Assays Wandbild Zellen
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