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Miniaturiserede prøveforberedelse til transmissions Elektron Mikroskopi
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Miniaturiserede prøveforberedelse til transmissions Elektron Mikroskopi

Article DOI: 10.3791/57310-v • 09:04 min • July 27th, 2018
July 27th, 2018 •
*1,2, *1, *1,2, 1, 1, 1, 1, 1, 3, 1, 1
1Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, 2Swiss Nanoscience Institute, University of Basel, 3BioEM lab, Biozentrum, University of Basel
* These authors contributed equally

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Et instrument og metoder til udarbejdelse af nanoliter mellemstore prøve diskenheder for transmissions Elektron Mikroskopi er præsenteret. Ingen papir-blotting trin er påkrævet, således at man undgår de skadelige konsekvenser dette kan have for proteiner, betydeligt reducere prøve tab og muliggør analysen af enkelt celle lysate for visuel proteomics.

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Biologi sag 137 elektronmikroskopi cryo negative pletten mikrofluidik prøve conditioning visuelle proteomics encellede lysis
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