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Developmental Biology

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JoVE Journal Developmental Biology
CRISPR-mediert tap av funksjon analyse i lillehjernen Granule celler ved hjelp av In Utero Electroporation-baserte genoverføring
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CRISPR-mediert tap av funksjon analyse i lillehjernen Granule celler ved hjelp av In Utero Electroporation-baserte genoverføring

Article DOI: 10.3791/57311-v • 09:39 min • June 9th, 2018
June 9th, 2018 •
*1, *2, 2, 3,4,5, 1, 3,4
1Division of Molecular Neurogenetics, German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, 2Division of Molecular Genetics, German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), 3Hopp-Children's Cancer Center at the NCT Heidelberg (KiTZ), 4Division of Pediatric Neurooncology, German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), 5Department of Pediatric Hematology and Oncology, Heidelberg University Hospital
* These authors contributed equally

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Konvensjonelle tap-av-funksjon studier av gener bruke knockout dyr er ofte kostbare og tidkrevende. Electroporation-baserte CRISPR-mediert somatisk mutagenese er et kraftig verktøy for å forstå gen fungerer i vivo. Her rapporterer vi en metode for å analysere knockout fenotyper i voksende celler av lillehjernen.

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Utviklingspsykologi biologi problemet 136 målrettet gen levering i utero electroporation lillehjernen GNP grobunn CRISPR/Cas9 hjernens utvikling hjernesvulster
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