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JoVE Journal Bioengineering
Menneskelige Pluripotent stamceller kultur på polyvinylacetat alkohol-Co-Itaconic syre Hydrogels med varierende stivhet Under Xeno-gratis forhold
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Menneskelige Pluripotent stamceller kultur på polyvinylacetat alkohol-Co-Itaconic syre Hydrogels med varierende stivhet Under Xeno-gratis forhold

Article DOI: 10.3791/57314-v • 11:37 min • February 3rd, 2018
February 3rd, 2018 •
*1, *1, 1,2, 3,4, 1, 1, 1, 2, 2, 5, 6, 7,8
1Department of Chemical and Materials Engineering, National Central University, 2Department of Botany and Microbiology, King Saud University, 3Cathay Medical Research Institute, Cathay General Hospital, 4Graduate Institute of Systems Biology and Bioinformatics, National Central University, 5Department of Medical Microbiology and Parasitology, Universiti Putra Malaysia, 6Department of Internal Medicine, Taiwan Landseed Hospital, 7Department of Zoology, Bharathiar University, 8Thiruvalluvar University
* These authors contributed equally

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En protokoll er presentert for å forberede polyvinylacetat alkohol-co-itaconic syre hydrogels med varierende stivhet, som ble podet med og uten oligopeptides, for å undersøke effekten av stivhet av biologisk materiale på differensiering og spredning stamceller. Stivhet av hydrogels ble kontrollert av crosslinking tiden.

Tags

Bioteknologi problemet 132 Hydrogel indusert pluripotent stamceller embryonale stamcelleforskningen elastisitet cellekultur oligopeptide overflate modifisering nanosegment
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