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JoVE Journal Bioengineering
Cultura de células-tronco pluripotentes humanas em polivinil álcool-Co-itacónico ácido hidrogel com rigidez diferentes condições Xeno-livre
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Cultura de células-tronco pluripotentes humanas em polivinil álcool-Co-itacónico ácido hidrogel com rigidez diferentes condições Xeno-livre

Article DOI: 10.3791/57314-v • 11:37 min • February 3rd, 2018
February 3rd, 2018 •
*1, *1, 1,2, 3,4, 1, 1, 1, 2, 2, 5, 6, 7,8
1Department of Chemical and Materials Engineering, National Central University, 2Department of Botany and Microbiology, King Saud University, 3Cathay Medical Research Institute, Cathay General Hospital, 4Graduate Institute of Systems Biology and Bioinformatics, National Central University, 5Department of Medical Microbiology and Parasitology, Universiti Putra Malaysia, 6Department of Internal Medicine, Taiwan Landseed Hospital, 7Department of Zoology, Bharathiar University, 8Thiruvalluvar University
* These authors contributed equally

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Um protocolo é apresentado para preparar polivinil álcool-co-itacónico ácido hidrogel com rigidez diferentes, que foram enxertados com e sem oligopeptídeos, para investigar o efeito da rigidez dos biomateriais na diferenciação e proliferação de células-tronco. A rigidez do hidrogel era controlada pelo tempo de reticulação.

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Bioengenharia questão 132 hidrogel induzida por células-tronco pluripotentes células-tronco embrionárias elasticidade cultura celular oligopeptídeos modificação da superfície nanosegment
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