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Journal / Bioengineering / Gel-seq: En metode til samtidige sekventering…
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Bioengineering

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3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
Journal (Bioengineering)
3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics
Journal (Bioengineering)
Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics
 
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Gel-seq: En metode til samtidige sekventering bibliotek forberedelse af DNA og RNA bruger Hydrogel matricer

Article DOI: 10.3791/57315-v • 09:19 min • March 26th, 2018
March 26th, 2018 •
*1, *2, 2, 3, 2
1Department of Engineering, University of San Diego, 2Department of Bioengineering, University of California, San Diego, 3Department of Mechanical and Aerospace Engineering, University of California, San Diego
* These authors contributed equally

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Gel-seq gør det muligt for forskere at samtidig forberede biblioteker til både DNA - og RNA-seq ubetydelige ekstra omkostninger fra 100-1000 celler ved hjælp af en simpel hydrogel enhed. Dette paper præsenterer en detaljeret tilgang til fabrikation af enheden samt den biologiske protokol til at generere parrede biblioteker.

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Bioteknologi sag 133 elektroforese polyacrylamid hydrogel næste generation sequencing genom transkriptom bibliotek forberedelse DNA RNA
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