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Journal / Biochemistry / Iluminando os caminhos para a ativação de Caspase …
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Iluminando os caminhos para a ativação de Caspase usando fluorescência Bimolecular complementação
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Iluminando os caminhos para a ativação de Caspase usando fluorescência Bimolecular complementação

Article DOI: 10.3791/57316-v • 08:47 min • March 5th, 2018
March 5th, 2018 •
1, 2
1Department of Pediatrics, Division of Hematology-Oncology, Baylor College of Medicine, 2Department of Pediatrics, Division of Hematology-Oncology and Department of Molecular and Cellular Biology, Baylor College of Medicine

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Este protocolo descreve caspase Bimolecular complementação de fluorescência (BiFC); um método baseado em imagens que pode ser usado para visualizar induzida proximidade das caspases de iniciador, que é o primeiro passo para sua ativação.

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Bioquímica edição 133 Caspase apoptose complementação Bimolecular de fluorescência microscopia Venus lapso de tempo
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