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Journal / Genetics / 3 ' 快速扩增 CDNA 末端, 以图记录癌症
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JoVE Journal Genetics
3 ' 快速扩增 CDNA 末端, 以图记录癌症
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3 ' 快速扩增 CDNA 末端, 以图记录癌症

Article DOI: 10.3791/57318-v • 09:38 min • March 28th, 2018
March 28th, 2018 •
1, 1
1Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Butler University

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在这里描述的两种不同的 3 ' 快速扩增的 cDNA 结束 (3 ' 种族) 协议使用两种不同的 DNA 聚合酶来映射序列, 包括开放阅读框架 (ORF) 的一部分, 停止密码子, 和整个 3 ' UTR 的成绩单使用 RNA从不同的癌细胞系获得的。

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遗传学 问题 133 成绩单 3 ' 末端 3 ' 迅速放大 cDNA 末端 (3 ' 种族) 聚合酶链反应 polyadenylation 信号 (PAS) polyadenylation
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