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JoVE Journal Genetics
Tilpasse 3' slutter hurtige forstærkning af CDNA for at kortlægge udskrifter i kræft
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Tilpasse 3' slutter hurtige forstærkning af CDNA for at kortlægge udskrifter i kræft

Article DOI: 10.3791/57318
March 28th, 2018 •
1, 1
1Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Butler University

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March 28th, 2018

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Click here for the English version.

De to forskellige 3' hurtige forstærkning af cDNA ender (3' RACE) protokoller beskrevet her gøre brug af to forskellige DNA polymeraser til at knytte sekvenser, der omfatter et segment af åben behandling ramme (ORF), stop-codon og den hele 3' UTR af et udskrift ved hjælp af RNA fremstillet af forskellige kræft cellelinjer.

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