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JoVE Journal Genetics
Tilpasse 3 slutter raske forsterkning av CDNA å tilordne utskrifter i kreft
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Tilpasse 3 slutter raske forsterkning av CDNA å tilordne utskrifter i kreft

Article DOI: 10.3791/57318-v • 09:38 min • March 28th, 2018
March 28th, 2018 •
1, 1
1Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Butler University

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To forskjellige 3' rask forsterkningen på cDNA ender (3' RACE) protokoller beskrevet her gjør bruk av to forskjellige DNA polymerases tilordne sekvenser som inkluderer et segment åpent lesing (ORF), stopp codon, og det hele 3' UTR av en utskrift ved hjelp av RNA Hentet fra ulike kreftcelle linjer.

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Genetikk problemet 133 utskrifter 3 slutten 3 raske forsterkning av cDNA ender (3' RACE) polymerasekjedereaksjons polyadenylation signal (PAS) polyadenylation
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