JoVE Journal
Bioengineering
Bioengineering
This content is Open Access.
Preparation, Purification, and Use of Fatty Acid-containing Liposomes
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Preparation, Purification, and Use of Fatty Acid-containing Liposomes
February 9th, 2018
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1Howard Hughes Medical Institute and Department of Molecular Biology and Center for Computational and Integrative Biology, Massachusetts General Hospital, 2Department of Biomedical Engineering, Boston University, 3Department of Genetics, Cell Biology, and Development, University of Minnesota
* These authors contributed equally
Chapters
Summary
Liposomes containing single-chain amphiphiles, particularly fatty acids, exhibit distinct properties compared to those containing diacylphospholipids due to the unique chemical properties of single chain amphiphiles. Here we describe techniques for the preparation, purification, and use of liposomes comprised in part or whole of these amphiphiles.
Tags
Liposomes Fatty Acids Preparation Purification Use Synthetic Biology Origin Of Life Studies Minimal Cells Artificial Protocells Protocol High Yield Monodispersed Experimental Conditions Phospholipid Vesicles Thin Films Dried Lipids Gas-tight Syringes Chloroform Nitrogen Argon Fume Hood House Vacuum Rehydration Buffer Sodium Hydroxide Concentration Vesicle Formation VortexerRelated Videos
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