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JoVE Journal
Biochemistry

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Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction
 
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Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction

Article doi: 10.3791/57334
February 15th, 2018

Summary February 15th, 2018

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A detailed protocol to perform a titration ELISA is described. Moreover, a novel algorithm is presented to evaluate titration ELISAs and to obtain a dissociation constant of binding of a soluble ligand to a microtiter plate-immobilized receptor.

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