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Journal / Immunology and Infection / Met behulp van Terminal Transferase-gemedieerde…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Met behulp van Terminal Transferase-gemedieerde dUTP testen Nick einde-etikettering (TUNEL) en Caspase-3/7 aan maatregel epidermale celdood in kikkers met Chytridiomycosis
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Met behulp van Terminal Transferase-gemedieerde dUTP testen Nick einde-etikettering (TUNEL) en Caspase-3/7 aan maatregel epidermale celdood in kikkers met Chytridiomycosis

Article DOI: 10.3791/57345-v • 07:37 min • May 16th, 2018
May 16th, 2018 •
1,2, 1, 1, 1
1One Health Research Group, College of Public Health, Medical and Veterinary Sciences, James Cook University, 2Department of Biological Sciences, University of Pittsburgh

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We kwantificeren epidermale celdood in kikkers met chytridiomycosis met twee methoden. Ten eerste, we gebruiken terminal transferase-gemedieerde dUTP nick einde-etikettering (TUNEL) in situ histologie om verschillen tussen klinisch besmette en niet-geïnfecteerde dieren te bepalen. Ten tweede, wij voeren een tijdreeksanalyse van apoptosis over infectie met behulp van een caspase-3/7 eiwit analyse.

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Immunologie en infectie uitgeven 135 Apoptosis Caspase histologie pathologie TUNEL Wildlife Disease
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