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Journal / Immunology and Infection / Bruke Terminal Transferase-mediert dUTP søk Nick…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Bruke Terminal Transferase-mediert dUTP søk Nick slutten-merking (tunnel) og Caspase 3/7 til mål Epidermal celledød i frosker med Chytridiomycosis
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Bruke Terminal Transferase-mediert dUTP søk Nick slutten-merking (tunnel) og Caspase 3/7 til mål Epidermal celledød i frosker med Chytridiomycosis

Article DOI: 10.3791/57345-v • 07:37 min • May 16th, 2018
May 16th, 2018 •
1,2, 1, 1, 1
1One Health Research Group, College of Public Health, Medical and Veterinary Sciences, James Cook University, 2Department of Biological Sciences, University of Pittsburgh

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Vi kvantifisere epidermal celledød i frosker med chytridiomycosis bruker to metoder. Først bruker vi terminal transferase-mediert dUTP nick slutten-merking (tunnel) i situ histology til å finne forskjellen klinisk infisert og smittet dyr. Andre gjennomføre vi en tidsserier analyse av apoptose over infeksjon med en caspase 3/7 protein analyse.

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Immunologi og infeksjon utstede 135 apoptose Caspase Histology patologi tunnel Wildlife sykdom
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