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Journal / Genetics / הגנום משופרת עריכה עם Cas9 Ribonucleoprotein…
JoVE Journal
Genetics

This content is Open Access.

JoVE Journal Genetics
הגנום משופרת עריכה עם Cas9 Ribonucleoprotein שונים תאים ואורגניזמים
Characterizing Mutational Load and Clonal Composition of Human Blood
Journal (Genetics)
Characterizing Mutational Load and Clonal Composition of Human Blood
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הגנום משופרת עריכה עם Cas9 Ribonucleoprotein שונים תאים ואורגניזמים

Article DOI: 10.3791/57350-v • 09:51 min • May 25th, 2018
May 25th, 2018 •
*1,2, *1, *3,4,5,6, *1,3, 1,3, 3,5,6,7,8,9, 1,2, 1,10, 3
1Department of Molecular Cell Biology, University of California, Berkeley, 2Howard Hughes Medical Institute, University of California, Berkeley, 3Innovative Genomics Institute, University of California, Berkeley, 4Biomedical Sciences Graduate Program, University of California, San Francisco, 5Department of Microbiology and Immunology, University of California, San Francisco, 6Diabetes Center, University of California, San Francisco, 7Chan Zuckerberg Biohub, 8Department of Medicine, University of California, San Francisco, 9UCSF Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, 10Department of Integrative Biology, University of California, Berkeley
* These authors contributed equally

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Click here for the English version.

ניצול של Cas9 preassembled ribonucleoprotein מורכבים (RNP) היא שיטה חזקה לעריכת הגנום מדויק, יעיל. כאן, אנחנו להדגיש השירות שלה על פני מגוון רחב של תאים, אורגניזמים, כולל תאים אנושיים ראשי שני קלאסי והעתידיים דגם אורגניזמים.

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גנטיקה גיליון 135 CRISPR Cas9 gRNA RNP הגנום עריכה עריכה ג'ין את-מטרות HDR HSPCs תאי T Caenorhabditis elegans Parhyale hawaiensis
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