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Journal / Genetics / Verbesserte Genom Bearbeitung mit Cas9…
JoVE Journal
Genetics

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JoVE Journal Genetics
Verbesserte Genom Bearbeitung mit Cas9 Ribonucleoprotein in verschiedenen Zellen und Organismen
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Verbesserte Genom Bearbeitung mit Cas9 Ribonucleoprotein in verschiedenen Zellen und Organismen

Article DOI: 10.3791/57350-v • 09:51 min • May 25th, 2018
May 25th, 2018 •
*1,2, *1, *3,4,5,6, *1,3, 1,3, 3,5,6,7,8,9, 1,2, 1,10, 3
1Department of Molecular Cell Biology, University of California, Berkeley, 2Howard Hughes Medical Institute, University of California, Berkeley, 3Innovative Genomics Institute, University of California, Berkeley, 4Biomedical Sciences Graduate Program, University of California, San Francisco, 5Department of Microbiology and Immunology, University of California, San Francisco, 6Diabetes Center, University of California, San Francisco, 7Chan Zuckerberg Biohub, 8Department of Medicine, University of California, San Francisco, 9UCSF Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, 10Department of Integrative Biology, University of California, Berkeley
* These authors contributed equally

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Unter Verwendung einer vormontierten Cas9 ist Ribonucleoprotein Komplex (RNP) eine leistungsfähige Methode für präzise und effiziente Genom-Bearbeitung. Hier heben wir ihre Nützlichkeit in einem breiten Spektrum von Zellen und Organismen, einschließlich der primären humanen Zellen und sowohl klassische und neue Modellorganismen.

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Ausgabe 135 CRISPR Cas9 Genetik gRNA RNP Genom-Bearbeitung gen zu bearbeiten sich Ziele HDR HSPCs T-Zellen Caenorhabditis Elegans Parhyale Hawaiensis
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