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Journal / Genetics / Forbedret genom-redigering med Cas9…
JoVE Journal
Genetics

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JoVE Journal Genetics
Forbedret genom-redigering med Cas9 Ribonucleoprotein i forskellige celler og organismer
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Forbedret genom-redigering med Cas9 Ribonucleoprotein i forskellige celler og organismer

Article DOI: 10.3791/57350-v • 09:51 min • May 25th, 2018
May 25th, 2018 •
*1,2, *1, *3,4,5,6, *1,3, 1,3, 3,5,6,7,8,9, 1,2, 1,10, 3
1Department of Molecular Cell Biology, University of California, Berkeley, 2Howard Hughes Medical Institute, University of California, Berkeley, 3Innovative Genomics Institute, University of California, Berkeley, 4Biomedical Sciences Graduate Program, University of California, San Francisco, 5Department of Microbiology and Immunology, University of California, San Francisco, 6Diabetes Center, University of California, San Francisco, 7Chan Zuckerberg Biohub, 8Department of Medicine, University of California, San Francisco, 9UCSF Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, 10Department of Integrative Biology, University of California, Berkeley
* These authors contributed equally

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Udnytter en hovedmeningen Cas9 er ribonucleoprotein komplekse (RNP) en kraftfuld metode for præcis, effektiv genom-redigering. Her, fremhæve vi sin nytte over en bred vifte af celler og organismer, herunder primære menneskeceller og både klassiske og nye modelorganismer.

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Genetik spørgsmålet 135 CRISPR Cas9 gRNA RNP genom-redigering redigering af genet off mål HDR HSPCs T-celler Caenorhabditis elegans Parhyale hawaiensis
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