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Journal / Genetics / Verbeterde genoom bewerken met Cas9…
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Genetics

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JoVE Journal Genetics
Verbeterde genoom bewerken met Cas9 Ribonucleoprotein in verschillende cellen en organismen
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Verbeterde genoom bewerken met Cas9 Ribonucleoprotein in verschillende cellen en organismen

Article DOI: 10.3791/57350-v • 09:51 min • May 25th, 2018
May 25th, 2018 •
*1,2, *1, *3,4,5,6, *1,3, 1,3, 3,5,6,7,8,9, 1,2, 1,10, 3
1Department of Molecular Cell Biology, University of California, Berkeley, 2Howard Hughes Medical Institute, University of California, Berkeley, 3Innovative Genomics Institute, University of California, Berkeley, 4Biomedical Sciences Graduate Program, University of California, San Francisco, 5Department of Microbiology and Immunology, University of California, San Francisco, 6Diabetes Center, University of California, San Francisco, 7Chan Zuckerberg Biohub, 8Department of Medicine, University of California, San Francisco, 9UCSF Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, 10Department of Integrative Biology, University of California, Berkeley
* These authors contributed equally

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Met behulp van een voorgemonteerd Cas9 is ribonucleoprotein complex (RNP) een krachtige methode voor het bewerken van nauwkeurige en efficiënte genoom. Hier, wijzen wij op het nut ervan over een breed scala van cellen en organismen, met inbegrip van de primaire menselijke cellen en beide klassieke en opkomende modelorganismen.

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Genetica kwestie 135 CRISPR Cas9 gRNA RNP genoom bewerken bewerken van gene af-doelen HDR HSPCs T-cellen Caenorhabditis elegans Parhyale hawaiensis
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