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Journal / Genetics / Génome améliorée d’édition avec Cas9…
JoVE Journal
Genetics

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JoVE Journal Genetics
Génome améliorée d’édition avec Cas9 ribonucléoprotéique dans diverses cellules et des organismes
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Génome améliorée d’édition avec Cas9 ribonucléoprotéique dans diverses cellules et des organismes

Article DOI: 10.3791/57350-v • 09:51 min • May 25th, 2018
May 25th, 2018 •
*1,2, *1, *3,4,5,6, *1,3, 1,3, 3,5,6,7,8,9, 1,2, 1,10, 3
1Department of Molecular Cell Biology, University of California, Berkeley, 2Howard Hughes Medical Institute, University of California, Berkeley, 3Innovative Genomics Institute, University of California, Berkeley, 4Biomedical Sciences Graduate Program, University of California, San Francisco, 5Department of Microbiology and Immunology, University of California, San Francisco, 6Diabetes Center, University of California, San Francisco, 7Chan Zuckerberg Biohub, 8Department of Medicine, University of California, San Francisco, 9UCSF Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, 10Department of Integrative Biology, University of California, Berkeley
* These authors contributed equally

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Utilisant un Cas9 préassemblé complexe ribonucléoprotéique (RNP) est une méthode puissante pour l’édition de génome précis et efficace. Ici, nous mettons en évidence son utilité dans un large éventail de cellules et des organismes, y compris des cellules humaines primaires et les deux classiques et de nouveaux organismes modèles.

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Numéro 135 CRISPR Cas9 génétique gRNA RNP modification du génome gène édition off-cibles HDR HSPCs lymphocytes T Caenorhabditis elegans Parhyale affinis
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