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Journal / Genetics / Forbedret genomet redigering med Cas9…
JoVE Journal
Genetics

This content is Open Access.

JoVE Journal Genetics
Forbedret genomet redigering med Cas9 Ribonucleoprotein i ulike celler og organismer
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Forbedret genomet redigering med Cas9 Ribonucleoprotein i ulike celler og organismer

Article DOI: 10.3791/57350-v • 09:51 min • May 25th, 2018
May 25th, 2018 •
*1,2, *1, *3,4,5,6, *1,3, 1,3, 3,5,6,7,8,9, 1,2, 1,10, 3
1Department of Molecular Cell Biology, University of California, Berkeley, 2Howard Hughes Medical Institute, University of California, Berkeley, 3Innovative Genomics Institute, University of California, Berkeley, 4Biomedical Sciences Graduate Program, University of California, San Francisco, 5Department of Microbiology and Immunology, University of California, San Francisco, 6Diabetes Center, University of California, San Francisco, 7Chan Zuckerberg Biohub, 8Department of Medicine, University of California, San Francisco, 9UCSF Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, 10Department of Integrative Biology, University of California, Berkeley
* These authors contributed equally

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Bruker en prefabrikkerte Cas9 er ribonucleoprotein kompleks (RNP) en kraftig metode for presis og effektiv genomet redigering. Her markere vi sin verktøyet over et bredt spekter av celler og organismer, inkludert primære menneskelige celler og både klassiske og nye modell organismer.

Tags

Genetikk problemet 135 CRISPR Cas9 gRNA RNP genomet redigering gene redigering av mål HDR HSPCs T-celler Caenorhabditis elegans Parhyale hawaiensis
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