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JoVE Journal
Immunology and Infection

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基于聚丙烯酰胺的多井格式实验研究细胞外基质硬度对黏附细胞细菌感染的影响
 
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基于聚丙烯酰胺的多井格式实验研究细胞外基质硬度对黏附细胞细菌感染的影响

Article DOI: 10.3791/57361-v 11:51 min July 5th, 2018
July 5th, 2018

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Summary

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Click here for the English version.

我们开发了一种多井格式的聚丙烯酰胺检测方法, 以探讨细胞外基质硬度对黏附细胞细菌感染的影响。该检测与流式细胞术、染色和牵引力显微镜相兼容, 可以定量测量细胞、胞外基质和致病细菌之间的生物力学相互作用。

Tags

免疫学和感染 问题 137 黏附哺乳动物细胞 细菌感染 内皮 流式细胞术 牵引力显微镜 聚丙烯酰胺水凝胶 染色 基底刚度
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