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JoVE Journal
Biology
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高通量 SiRNA 筛选氯化苦和氟化氢引起的角膜上皮细胞损伤
 
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高通量 SiRNA 筛选氯化苦和氟化氢引起的角膜上皮细胞损伤

Article DOI: 10.3791/57372-v 14:20 min June 16th, 2018
June 16th, 2018

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Summary

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高通量小抑制 RNA 筛选是一种重要的工具, 有助于更迅速地阐明化学角膜上皮损伤的分子机制。本文对氟化氢-氯化苦诱导的角膜上皮损伤进行高通量筛选的暴露模型和方法进行了开发和验证。

Tags

生物学 问题 136 氯化苦 氟化氢 角膜上皮细胞 角膜损伤 siRNA 高通量筛选
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