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JoVE Journal Cancer Research
Hög känslighet detektion av Micrometastases genereras av GFP Lentivirus-sensorik Organoids odlade från en patientderiverade kolon tumör
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A2780 Cells Genomic DNA Isolation and Analysis (Video) | JoVE
Journal (Cancer Research)
A2780 Cells Genomic DNA Isolation and Analysis (Video) | JoVE
 
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Hög känslighet detektion av Micrometastases genereras av GFP Lentivirus-sensorik Organoids odlade från en patientderiverade kolon tumör

Article DOI: 10.3791/57374-v • 09:16 min • June 14th, 2018
June 14th, 2018 •
1,2, 1,2, 2,4, 5, 1, 1, 1, 3, 2, 1, 2
1Department of Coloproctological Surgery, Juntendo University Faculty of Medicine, 2Department of Molecular Pathogenesis, Juntendo University Faculty of Medicine, 3Atopy Research Center, Juntendo University Faculty of Medicine, 4Department of Oral Pathobiological Science and Surgery, Tokyo Dental College, 5Department of Gastroenterology, Juntendo University Faculty of Medicine

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För att möjliggöra mycket känslig detektion av sprida mänskliga kolorektal cancer (CRC) celler kolonisera vävnader, visar vi häri ett protokoll för effektiv transduktion av grönt fluorescerande protein (GFP) lentiviral partiklar i PDX-derived CRC organoid celler före sin injektion i mottagarens möss, med stereo-fluorescens mikroskopisk observation.

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Cancerforskning fråga 136 mänskliga CRC patientderiverade tumör xenograft (PDXs) GFP-märkta PDX-derived tumör organoids lentiviral transduktion micrometastasis 3D kultur
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